トップ＞メンバー紹介＞千坂先生について

千坂先生研究内容紹介

• 研究名　Cornichon-like protein facilitates secretion of HB-EGF and regulates proper development of cranial nerves.　 （コルニション類似タンパクはHB-EGFの分泌を促進し、鰓弓神経形成を制御する。）

• 研究内容 Hoshino, H., Uchida ,T., Otsuki, T., Kawamoto, S., Okubo, K., Takeichi, M., and Chisaka, O. Mol Biol Cell. 18, 1143-1152. (2007)
  末梢への移動過程において、頚部神経堤細胞は菱脳分節３、５の側方間充織でErbB4依存的に排斥される。ErbB4のリガンドは多く、実際にどのリガンドが上記機構に関与するか不明であった。今回我々はコルニション類似タンパクがニワトリ胚菱脳分節３、５に発現することを発見し、培養細胞系ではコルニション類似タンパクはErbB4リガンドの一つであるHB-EGFの分泌を促進することを明らかにした。カルボキシ末端を欠いたコルニション類似タンパクをニワトリ胚で強制発現すると神経堤細胞の分布が異常になり、鰓弓神経節間に異常な軸索連絡が生じる。siRNAによるコルニション類似タンパクやHB-EGFの発現抑制でも類似の表現型が見られた。HB-EGFは菱脳では広範囲に発現しており、コルニション類似タンパクはHB-EGFの機能をErbB4とともに菱脳分節３、５に限局する働きをしていると思われる。

千坂先生発表論文

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• Kuge, S., Chisaka, O., et al. （6人中2番目）(1984)
  Expression of poliovirus complementary DNA coding for viral antigenic determinants in　Escherichia coli. J. Biochem. (Tokyo) 96, 305-312

• Tokunaga, T., Iwai, S., et al. （８人中７番目）(1984)
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• Chisaka, O., Iwai, S., Ohtsuka, E., and Matsubara, K (1986)
  Cloning a defined region of DNA using a limited action of DNA polymerase: application to dissection of hepatitis B virus surface antigene gene. Gene 45, 19-25

• Chisaka, O., Araki, K., et al. (7人中１番目) (1987)
  Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in　a human hepatoblastoma cell line producing hepatitis B virus. Gene 60, 183-189

• Ueda, K., Tsurimoto, T., Nagahata, T., Chisaka, O., and Matsubara, K. (1989)
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• Katayama, K., Hayashi, N., et al. (10人中8番目) (1989)
  Detection of hepatitis B virus X gene protein and antibody in type B chronic liver disease. Gastroenterology 97, 990-998

• Nagahata, T, Ueda, K., Tsurimoto, T., Chisaka, O., and Matsubara, K. (1989)
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• Araki, K., Shiosaki, K. Araki, M., Chisaka, O., and Matsubara, K. (1990)
  The essential region for assembly and particle formation in hepatitis B virus surface antigen produced in yeast cells. Gene 89, 195-201

• Kawamoto, S., Yamamoto, S., Ueda, K., Nagahata, T., Chisaka, O., and Matsubara, K. (1990)
  Translation of hepatitis B virus DNA polymerase from their internal AUG codon, not from the upstream AUG codon for the core protein. Biochem. Biophys. Res. Commun. 171, 1130-1136

• Chisaka, O. and Capecchi, M. R. (1991)
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• Chisaka, O., Musci, T. M., and Capecchi, M. R. (1992)
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• Kamogawa, Y., Yamauchi, K., Obata, H., Chisaka, O., and Matsubara, K. (1992)
  Hepatitis B virus-DNA transfected myeloma cell-specific cytotoxic T cells in chronic Hepatitis B patients. Virology 191, 321-326

• Nakatake, H., Chisaka, O., Yamamoto, S., Matsubara, K., and Koshy, R. (1993)
  Effect of X protein on transactivation of hepatitis B virus promoters and on viral replication. Virology 195, 305-314

• Carpenter, E. M., Goddard, J. M., Chisaka, O., Manley. N. R., and Capecchi, M. R. (1993)
  Loss of Hox-A1(Hox-1.6) function results in the reorganization of the murine hindbarin. Development 118, 1063-1075

• Inoue, T., Chisaka, O., Matsunami, H., and Takeichi, M. (1997)
  Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos. Dev. Biol. 183, 183-194

• Li., Z. L., Chisaka, O., Koseki, H., Akasaka, T., Ishibasi, M., and Shiota, K. (1997)
  Heat shock -induced homeotic transformations of the axial skeleton and associated shifts of Hox gene expression domains in mouse embryos. Reprod. Toxicol. 11, 761-770

• Fujimoto, S., Araki, K., Chisaka, O., Araki, M., Takagi, K., and Yamamura, K. (1998)
  Analysis of the murine Hoxa-9 cDNA: an alternatively spliced transcript encodes a truncated protein lacking the homeodomain. Gene 209, 77-85

• Hashimoto, J., Murakami, G., Tsugane, M. H., Chisaka, O., Capecchi, M. R., and Ogino, (1999)
  Lumbosacral plexsus in Hoxa9 knockout mice with special reference to their nerve variations identified according to whether they were interphenotypic or intergenotypic differences. Kaibougaku Zasshi 74, 609-630

• Horikawa, K., Radice, G., Takeichi, M., and Chisaka, O. (1999)
  Adhesive subdivisions intrinsic to epithelial somites. Dev. Biol. 215, 182-189

• Kawamoto, S., Ohnishi, T., Kita, H., Chisaka, O., and Oukubo, K. (1999)
  Expression profiling by iAFLP: a PCR-based method for genome-wide geneexpression profiling. Genome Res. 9, 1305-1312

• Manabe, T., Togashi, H., et al. （10人中10番目）(2000)
  Loss of cadherin-11 adhesion receptor enhances plastic changes in hippocampal synapses and modifies behavioral responses. Molec. Cell. Neurosci. 15, 534-546

• Inoue, T., Tanaka, T.,Chisaka, O., Takeichi, M., Nakamura, S., and Osumi, N. (2001)
  Roles of cadherins in maintaining the compartment boundary between the cortex and striatum during development. Development 128, 561-569

• Kawaguchi, J., Azuma, Y., et al. (10人中9番目) (2001)
  Targeted disruption of cadherin-11 leads to a reduction in bone density in calvaria. J. Bone Miner. Res. 16, 1265-71

• Watari, N., Kameda, Y., Takeichi, M., and Chisaka, O. (2001)
  Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Dev. Biol. 240, 15-31

• Shima, Y., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Chisaka, O., Takeichi, M., and Uemura, T. (2002) Differential expression of the seven-pass transmembrane cadherin genes Celsr1-3 and distribution of the Celsr2 protein during mouse development Dev. Dyn. 223, 321-332

• Takebayashi, H., Ohtsuki, T., et al. （９人中8番目）(2002)
  Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube. Mech. Dev. 113, 169-174

• Kameda, Y., Nishimaki, T., takeichi, M., and Chisaka, O. (2002)
  Homeobox gene Hoxa3 is essential for the formation of the carotid body in the mouse embryos. Dev. Biol. 247, 197-209

• Takebayashi, H., Nabeshima, Y., Yoshida, S., Chisaka, O., Ikenaka, K., and Nabeshima, Y. The basic helix-loop-helix factor Olig2 is essential for development of motoneuron and oligodendrocyte lineages. (2002)
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• Togashi, H., Abe, K., Mizoguchi, A., Takaoka, K., Chisaka, O., and Takeichi, M. (2002)
  Cadherin regulates dendritic spine morphogenesis. Neuron 35, 77-89

• Kameda, Y., Watari-Goshima, N., Nishimaki, T. and Chisaka, O. (2003)
  Disruption of the Hoxa3 homeobox gene results in anomalies of the carotid artery system and the arterial baroreceptors. Cell Tissue Res. 311, 343-355

• Abe, K., Chisaka, O., Van Roy, F., and Takeichi, M. (2004)
  Stability of dendritic spines and synaptic contacts is controlled by alphaN-catenin. Nature Neurosci. 7, 357-363.

• Kameda, Y., Arai, Y., Nishimaki, T., and Chisaka, O. (2004)
  The role of Hoxa3 gene in parathyroid gland organogenesis of the mouse. J. Histochem. Cytochem. 52, 641-651

• Chisaka, O. and Kameda, Y. (2005)
  Hoxa3 regulates the proliferation and differentiation of the third pharyngeal arch mesenchyme in mice. Cell Tissue Res. 320, 77-89.

• Maekawa, M., Yamamoto, T., Tanoue, T., Yuasa, Y., Chisaka, O., and Nishida E.(2005)
  Requirement of the MAP kinase signaling pathways for mouse preimplantation development. Development 132, 1773-83.

• Kodama, T., Imai, H., Doi, T., Chisaka, O. Shichida, Y., and Fujiyoshi, Y. (2005)
  Expression and localization of an exogenous G proten-coupled receptor fused with the rhodopsin C-terminal sequence in the retinal rod cells of knockin mice. Experimental Eye Res. 80, 859-869.

• Imai, H., Kuwayama, S., Onishi, A., Morizumi, T., Chisaka, O., and Shichida, Y. (2005)
  Molecular properties of rod and cone visual pigments from purified chicken cone pigments to mouse rhodopsin in situ. Photochem Photobiol Sci. 667-674.

• Ding, L., Takebayashi, H., Watanabe, K., Ohtsuki, T., Tanaka, K. F., Nabeshima, Y., Chisaka, O., Ikenaka, K., and Ono, K. (2005)
  Short-term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord. Dev Dyn. 234, 622-632.

• Onishi, A., Hasegawa, J., Imai, H., Chisaka, O., Ueda, Y., Honda, Y., Tachibana, M., and Shichida, Y. (2005)
  Generation of knock-in mice carrying third cones with spectral sensitivity different from S and L cones. Zool. Sci. 22, 1145-1156.

• Imai, H., Kefalov, V., Sakurai, K., Chisaka , O., Ueda, Y., Onishi, A., Morizumi, T., Fu, Y., and Ichikawa, K., Nakatani K, Honda Y, Chen J, Yau KW, Shichida Y. (2007)
  Molecular properties of rhodopsin and rod function. J Biol Chem. 282, 6677-6684.

• Hoshino, H., Uchida ,T., Otsuki, T., Kawamoto, S., Okubo, K., Takeichi, M., and Chisaka, O. (2007)
  Cornichon-like protein facilitates secretion of HB-EGF and regulates proper development of cranial nerves. Mol Biol Cell. 18, 1143-1152.

• Lee, D. M., Kiener, H. P., Agarwal, S. K., Noss, E. H., Watts, G. F., Chisaka, O., Takeichi, M., andBrenner, M. B. (2007)
  Cadherin-11 in synovial lining formation and pathology in arthritis. Science. 315, 1006-1010.

• Kameda, Y., Nishimaki, T., and Chisaka, O. (2007)
  Expression of the Epithelial marker E-Cadherin by the Thyroid C Cells and Their Precursors during Murine Development J. Histochem. Cytochem. 55, 1075-1088.

• Kousaka, K., Kiyonari, H., Oshima, N., Nagafuchi, A., Shima, Y., Chisaka, O., and Uemura T. (2008)
  Genesis. 46,246-255.

• Schwenk, J., Harmel, N., Zolles, G., Bildl, W., Kulik, A., Heimrich, B., Chisaka, O., Jonas, P., Schulte, U., Fakler, B., and Klocker, N. (2009)
  Science. 323, 1313-1319.